Peptide vaccination using nonionic block copolymers induces protective anti-viral CTL responses
Identifieur interne : 003921 ( Main/Exploration ); précédent : 003920; suivant : 003922Peptide vaccination using nonionic block copolymers induces protective anti-viral CTL responses
Auteurs : J. Gibson Lanier [États-Unis] ; Mark J. Newman [États-Unis] ; Eushin M. Lee [États-Unis] ; Alessandro Sette [États-Unis] ; Rafi Ahmed [États-Unis]Source :
- Vaccine [ 0264-410X ] ; 2000.
English descriptors
- Teeft :
- Adjuvant, Ahmed, Antibody responses, Assay, Block copolymers, Cell response, Cell responses, Choriomeningitis, Chronic infection, Clone, Copolymer, Cytotoxic, Elispot, Elispot assay, Emulsion, Epitope, Gibson lanier, Helper peptide, Immune responses, Immunization, Immunol, Lanier, Lcmv, Lymphocyte, Lymphocytic, Lymphocytic choriomeningitis virus, Lysis, Mice vaccinated, Mouse, Multiple emulsion, Naive mice, Nonionic, Nonionic block copolymers, Peptide, Peptide vaccination, Persistent infection, Pharmingen, Plaque assay, Secreting, Secreting cells, Spleen, Spleen cells, Splenocytes, Target cells, Vaccinated, Vaccinated mice, Vaccine, Viral, Viral challenge, Viral infection, Virol.
Abstract
Abstract: High molecular weight nonionic block copolymers have been developed as vaccine adjuvants. We employed these adjuvants in water-in-oil emulsion and multiple emulsion formulations with a synthetic peptide-based antigen vaccine to test their ability to prime anti-viral CD8+ T cell responses. Vaccines were made using the H-2d-restricted immunodominant peptide from lymphocytic choriomeningitis virus (LCMV), NP118–126, and administered to BALB/c ByJ (H-2d) mice. Peptide-containing emulsions were able to induce NP118–126 specific CTL and IFN-γ secreting CD8+ T cells in the vaccinated mice and these responses were maintained for at least 90 days post immunization. At all times, the responses induced by the copolymer formulations were equal to, or better than, formulations based on incomplete Freund's adjuvant (IFA). In addition, the responses induced by prophylactic vaccination using the multiple emulsion formulation resulted in accelerated viral clearance following infection with a strain of LCMV (clone 13) that causes a persistent infection in naı̈ve adult mice. These results indicate that peptide vaccination using a formulation based on high molecular weight nonionic block copolymer in a simple water-in-oil or a multiple emulsion format can induce virus-specific CD8+ T cell responses and confer protection sufficient enough to prevent the establishment of a persistent infection.
Url:
DOI: 10.1016/S0264-410X(99)00220-0
Affiliations:
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Gibson Lanier</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Emory Vaccine Center and Department of Microbiology and Immunology, Emory University School of Medicine, G211 Rollins Research Center, 1510 Clifton Rd., Atlanta, GA 30322</wicri:regionArea><wicri:noRegion>GA 30322</wicri:noRegion></affiliation></author><author><name sortKey="Newman, Mark J" sort="Newman, Mark J" uniqKey="Newman M" first="Mark J" last="Newman">Mark J. Newman</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Vaxcel Inc., 154 Technology Parkway, Norcross, GA 30092</wicri:regionArea><wicri:noRegion>GA 30092</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Eushin M" sort="Lee, Eushin M" uniqKey="Lee E" first="Eushin M" last="Lee">Eushin M. Lee</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Vaxcel Inc., 154 Technology Parkway, Norcross, GA 30092</wicri:regionArea><wicri:noRegion>GA 30092</wicri:noRegion></affiliation></author><author><name sortKey="Sette, Alessandro" sort="Sette, Alessandro" uniqKey="Sette A" first="Alessandro" last="Sette">Alessandro Sette</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Epimmune Inc., 6555 Nancy Ridge Rd., Suite 200, SanDiego, CA 92121</wicri:regionArea><wicri:noRegion>CA 92121</wicri:noRegion></affiliation></author><author><name sortKey="Ahmed, Rafi" sort="Ahmed, Rafi" uniqKey="Ahmed R" first="Rafi" last="Ahmed">Rafi Ahmed</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Emory Vaccine Center and Department of Microbiology and Immunology, Emory University School of Medicine, G211 Rollins Research Center, 1510 Clifton Rd., Atlanta, GA 30322</wicri:regionArea><wicri:noRegion>GA 30322</wicri:noRegion></affiliation><affiliation></affiliation><affiliation wicri:level="1"><country wicri:rule="url">États-Unis</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Vaccine</title><title level="j" type="abbrev">JVAC</title><idno type="ISSN">0264-410X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000">2000</date><biblScope unit="volume">18</biblScope><biblScope unit="issue">5–6</biblScope><biblScope unit="page" from="549">549</biblScope><biblScope unit="page" to="557">557</biblScope></imprint><idno type="ISSN">0264-410X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0264-410X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Adjuvant</term><term>Ahmed</term><term>Antibody responses</term><term>Assay</term><term>Block copolymers</term><term>Cell response</term><term>Cell responses</term><term>Choriomeningitis</term><term>Chronic infection</term><term>Clone</term><term>Copolymer</term><term>Cytotoxic</term><term>Elispot</term><term>Elispot assay</term><term>Emulsion</term><term>Epitope</term><term>Gibson lanier</term><term>Helper peptide</term><term>Immune responses</term><term>Immunization</term><term>Immunol</term><term>Lanier</term><term>Lcmv</term><term>Lymphocyte</term><term>Lymphocytic</term><term>Lymphocytic choriomeningitis virus</term><term>Lysis</term><term>Mice vaccinated</term><term>Mouse</term><term>Multiple emulsion</term><term>Naive mice</term><term>Nonionic</term><term>Nonionic block copolymers</term><term>Peptide</term><term>Peptide vaccination</term><term>Persistent infection</term><term>Pharmingen</term><term>Plaque assay</term><term>Secreting</term><term>Secreting cells</term><term>Spleen</term><term>Spleen cells</term><term>Splenocytes</term><term>Target cells</term><term>Vaccinated</term><term>Vaccinated mice</term><term>Vaccine</term><term>Viral</term><term>Viral challenge</term><term>Viral infection</term><term>Virol</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: High molecular weight nonionic block copolymers have been developed as vaccine adjuvants. We employed these adjuvants in water-in-oil emulsion and multiple emulsion formulations with a synthetic peptide-based antigen vaccine to test their ability to prime anti-viral CD8+ T cell responses. Vaccines were made using the H-2d-restricted immunodominant peptide from lymphocytic choriomeningitis virus (LCMV), NP118–126, and administered to BALB/c ByJ (H-2d) mice. Peptide-containing emulsions were able to induce NP118–126 specific CTL and IFN-γ secreting CD8+ T cells in the vaccinated mice and these responses were maintained for at least 90 days post immunization. At all times, the responses induced by the copolymer formulations were equal to, or better than, formulations based on incomplete Freund's adjuvant (IFA). In addition, the responses induced by prophylactic vaccination using the multiple emulsion formulation resulted in accelerated viral clearance following infection with a strain of LCMV (clone 13) that causes a persistent infection in naı̈ve adult mice. These results indicate that peptide vaccination using a formulation based on high molecular weight nonionic block copolymer in a simple water-in-oil or a multiple emulsion format can induce virus-specific CD8+ T cell responses and confer protection sufficient enough to prevent the establishment of a persistent infection.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Gibson Lanier, J" sort="Gibson Lanier, J" uniqKey="Gibson Lanier J" first="J" last="Gibson Lanier">J. Gibson Lanier</name></noRegion><name sortKey="Ahmed, Rafi" sort="Ahmed, Rafi" uniqKey="Ahmed R" first="Rafi" last="Ahmed">Rafi Ahmed</name><name sortKey="Ahmed, Rafi" sort="Ahmed, Rafi" uniqKey="Ahmed R" first="Rafi" last="Ahmed">Rafi Ahmed</name><name sortKey="Lee, Eushin M" sort="Lee, Eushin M" uniqKey="Lee E" first="Eushin M" last="Lee">Eushin M. Lee</name><name sortKey="Newman, Mark J" sort="Newman, Mark J" uniqKey="Newman M" first="Mark J" last="Newman">Mark J. Newman</name><name sortKey="Sette, Alessandro" sort="Sette, Alessandro" uniqKey="Sette A" first="Alessandro" last="Sette">Alessandro Sette</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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